The replacement cycle for bottle brushes varies depending on the material. It is recommended to replace sponge bottle brushes every 1 to 2 months, and nylon bottle brushes every 2 to 3 months; high-quality nylon brushes may be used for up to six months.
You should replace a bottle brush immediately if the bristles begin to fall out or become damaged, if the brush develops an unpleasant odor, or if it becomes difficult to clean.
For maintenance, nylon bottle brushes can be sterilized using high-heat methods, such as boiling or steam treatment. Sponge bottle brushes, however, must be kept dry and well-ventilated; they should be washed immediately after each use and stored in a dry, airy location to prevent prolonged immersion in water.

